PGD is multi-stage procedure and each sample is transferred multiple times between tubes and each sample’s ID is reidentified multiple times. Misplacement or misidentification cannot be excluded. PGD NGS is the only technique in which sample delivered to INVICTA Genetic Laboratory is marked with the barcode on a molecular level. In each sample, after cell lysis, DNA molecules are labeled with a unique molecular barcode, which is recognized during sequencing stage. PGD NGS is absolutely not affected by misidentification.
During multi-stage processes safety of sample identification may be compromised due to simple misidentification or misplacement of the sample – a so called “human error”. This problem could possible by solved by automatization where human involvement, along with human error risk, is minimized. However, such automated systems can be costly and difficult to implement making their accessibility limited. Even such systems can only minimize the risk, but not exclude it completely. That’s simply not good enough for PGD.
Experts from INVICTA Genetic Laboratory have developed a unique protocol in which every sample delivered to our laboratory remains in its original tube until the molecular barcoding. First transfer from the original tube is only performed after molecular barcoding. That way any following steps in the process cannot by affected by misidentification.
Molecular barcode is the 10 or 12 letter code incorporated within the library adapter – the oligonucleotide ligated to DNA molecule during NGS library preparation process. This code gives at least 262144 possible IDs (in the last position is always letter C). Typically no more than 96 IDs are used in the laboratory, making it easy to establish significantly different versions of barcodes. In consequence, even if one or two letters were to be miscalled during sequencing, there is no chance that barcode sequence would be misclassified.
After sequencing, the results are reported based on molecular barcodes further improving sample identification safety. Features like “macro” IDs or location does not play any role in identification. In this way issues of human error due to misidentification or misplacement became irrelevant in this process. PGS NGS is the only technique in which this type of risk is totally excluded and entire process is no affected by potential misidentification and the results are always reported accurately.
PGD NGS from INVICTA Genetic Laboratory makes your samples safer than ever!